產(chǎn)品描述
Recombinant Human Matrix Metalloproteinase-1 is produced by our Mammalian expression system and the target gene encoding Phe20-Asn469 is expressed with a 6His tag at the C-terminus.The proenzyme needs to be activated by APMA for an activated form.
蛋白編號(hào)
P03956
產(chǎn)品別稱
Interstitial Collagenase; Fibroblast Collagenase; Matrix Metalloproteinase-1; MMP-1; MMP1; CLG
分子量
52.88 KDa
表觀分子量
56 KDa, reducing conditions
劑型描述
Supplied as a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 2mM CaCl2, 1mM DTT, 0.05%Brij35, 10% Glycerol, pH 5.0.
內(nèi)毒素
Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
純度-SDS-PAGE
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
保存條件
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
運(yùn)輸條件
The product is shipped on ice packs.
Upon receipt, store it immediately at the temperature listed below.
產(chǎn)品背景
Matrix Metalloproteinase-1 (MMP-1) is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 contains several distinct domains: a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin like) domain. MMP-1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, α1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL1, IGFBP3, IGFBP5, pro-MMP2, and pro-MMP9. A significant role of MMP1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into 3/4, 1/4 fragments. MMP1 may also be involved in enzyme cascades, cytokine regulation and cell surface molecule modulation.